Permanent Marker Chromatography

Exploring color separation or chromatography is a fun and engaging chemistry activity.  It can be done in many different ways.  This lab is for older students and helps them learn about materials that are soluble in liquids other than water.  


What is Chromatography?

Chromatography basically means “to write with colors.”  It is the process of separating a mixture by passing it through a medium in which the different components of the mixture move at different rates.  Russian botanist M.S. Tsewtt discovered paper chromatography in 1903.  He was able to separate the pigments in plants.  Paper chromatography is now used to determine the purity and makeup of many compounds.  In paper chromatography, the mixture is dissolved in a liquid, and the medium it will travel through is the filter paper.  How well the pigments separate is dependent on how well they travel up the paper and how soluble they are in the solvent.   

Permanent Marker Chromatography


You are probably thinking you can’t separate permanent markers.  They are permanent.  With the right medium, you can separate the pigments in permanent markers.  It is fun to see what colors the manufacturers use to make many different colors.  Students will enjoy this fun chemistry experiment.

Safety Precautions


The following activity uses nail polish remover, which contains acetone.  It has a high odor.  It should be used in a well-ventilated area.  This can be done in the classroom as a demonstration in front of the class.  It could also be done on a nice day outside.  Students could set them up on picnic tables.  Just make sure it is not windy.  Good lab practices should be followed.  Follow your school district's rules and keep material safety data sheets on file.

Blue had some other pigments, but it was mostly the primary color of blue.



Since yellow is also a primary color, it did not separate into other pigments.



Black always has lots of color separation.  Black is a combination of all the primary colors.  Look closely; you will see different shades of reds, blues, and yellows. 





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